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[KO Validated] DFFA Rabbit Polyclonal Antibody (CAB1032)

SKU:
CAB1032
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • KO Validated Antibodies 1 Anti-DFFA Antibody CAB1032KO Validated
  • KO Validated Antibodies 1 Anti-DFFA Antibody CAB1032KO Validated
  • KO Validated Antibodies 1 Anti-DFFA Antibody CAB1032KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] DFFA Rabbit Polyclonal Antibody
SKU:CAB1032
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).
Sequence:MEVT GDAG VPES GEIR TLKP CLLR RNYS REQH GVAA SCLE DLRS KACD ILAI DKSL TPVT LVLA EDGT IVDD DDYF LCLP SNTK FVAL ASNE KWAY NNSD GGTA WISQ ESFD VDET DSGA GLKW KNVA RQLK EDLS SIIL LSEE DLQM LVDA PCSD LAQE LRQS CATV QRLQ HTLQ QVLD QREE VRQS KQLL QLYL QALE KEGS LLSK QEES KAAF GEEV DAVD TGIS RETS SDVA LASH ILTA LREK QAPE LSLS SQDL ELVT KEDP KALA VALN WDIK KTET VQEA CERE LALR LQQT QSLH SLRS ISAS KASP PGDL QNPK RARQ DPT
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100
Synonyms:DFF1; ICAD; DFF-45; FA
Positive Sample:Jurkat,H460,HepG2,Mouse brain,Rat brain
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:37kDa
Observed MW:35kDa, 45kDa

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:1676
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] DFFA Rabbit pAb (CAB1032) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.

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