[KO Validated] ECH1 Rabbit Polyclonal Antibody (CAB19868)

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SKU:
CAB19868
€129 - €349

Description

Technical Manual

[KO Validated] ECH1 Rabbit Polyclonal Antibody

Product Name:[KO Validated] ECH1 Rabbit Polyclonal Antibody
Product SKU:CAB19868
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Immunogen:Recombinant protein of human ECH1.
Sequence:

Email for sequence

Gene ID:1891
Swiss Prot:Q13011
Synonyms:ECH1; HPXEL
Calculated MW:35kDa
Observed MW:32KDa
Reactivity:Human, Mouse, Rat
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry 
Positive Samples:293T
Cellular Location:Mitochondrion, Peroxisome

Product Images

[KO Validated] ECH1 Rabbit Polyclonal Antibody (CAB19868)

Western blot analysis of extracts from normal (control) and ECH1 knockout (KO) 293T cells, using ECH1 antibody (CAB19868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Uniprot Information

UniProt Protein Function:ECH1: Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4- trans-dienoyl-CoA. Belongs to the enoyl-CoA hydratase/isomerase family.
UniProt Protein Details:

Protein type:Mitochondrial; Isomerase; Amino Acid Metabolism - tyrosine; EC 5.3.3.-

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: membrane

Molecular Function:protein binding; receptor binding

NCBI Summary:This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]
UniProt Code:Q13011
NCBI GenInfo Identifier:82654933
NCBI Gene ID:1891
NCBI Accession:Q13011.2
UniProt Secondary Accession:Q13011,Q8WVX0, Q96EZ9, A8K745,
UniProt Related Accession:Q13011
Molecular Weight:35,816 Da
NCBI Full Name:Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
NCBI Synonym Full Names:enoyl-CoA hydratase 1
NCBI Official Symbol:ECH1 
NCBI Official Synonym Symbols:HPXEL 
NCBI Protein Information:delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Protein Name:Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Gene Name:ECH1 
UniProt Entry Name:ECH1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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