The [KO Validated] EEF1B2 Antibody (CAB6580) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has been validated for use in Western blot applications and demonstrates a high degree of specificity and sensitivity in detecting EEF1B2 in human samples.EEF1B2 is a key component of the eukaryotic translation elongation factor complex and plays a critical role in the elongation phase of protein synthesis. Dysregulation of EEF1B2 has been implicated in various diseases, including cancer and neurodegenerative disorders, making it an important target for research in these fields.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] EEF1B2 Antibody
SKU:
CAB6580
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.
Purification Method
Affinity purification
Gene ID
1933
RRID
AB_2863531
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] EEF1B2 Rabbit pAb (CAB6580) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and EEF1B2 knockout (KO) 293T cells, using [KO Validated] EEF1B2 Rabbit pAb (CAB6580) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using [KO Validated] EEF1B2 Rabbit pAb (CAB6580) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using [KO Validated] EEF1B2 Rabbit pAb (CAB6580) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
