The [KO Validated] HB-EGF Monoclonal Antibody (CAB11657) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed using rabbit-derived cells, is highly specific and sensitive for detecting HB-EGF in human samples.Validated for use in Western blot applications, this antibody binds specifically to the HB-EGF protein, allowing for precise detection and analysis in a variety of cell types. Its high reactivity and specificity make it an ideal choice for studies in cell biology, signal transduction, and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] HB-EGF Monoclonal Antibody
SKU:
CAB11657
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0663
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DTR, DTS, DTSF, HEGFL, HB-EGF
Positive Sample:
SK-OV-3, Mouse lung, Mouse spleen, Rat spleen
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein, Extracellular Space.
Calculated MW:
23kDa
Observed MW:
18kDa/21kDa/27kDa
Enables growth factor activity and heparin binding activity. Involved in several processes, including epidermal growth factor receptor signaling pathway; positive regulation of protein kinase B signaling; and positive regulation of wound healing. Located in cell surface and extracellular space. Implicated in glomerulosclerosis and perinatal necrotizing enterocolitis.
Purification Method
Affinity purification
Gene ID
1839
RRID
AB_2861621
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HB-EGF Rabbit mAb (CAB11657) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
