Anti-HMGCR Antibody (CAB16876)

Antibody Name:	Anti-HMGCR Antibody [KO Validated]
Antibody SKU:	CAB16876
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human HMGCR.

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, K-562

Immunogen:	Recombinant protein of human HMGCR.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3156
Uniprot:	P04035
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	92kDa/97kDa/99kDa
Observed MW:	110kDa

	Western blot analysis of extracts from normal (control) and HMGCR knockout (KO) 293T cells, using HMGCR antibody (CAB16876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of K-562 cells, using HMGCR Rabbit pAb (CAB16876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of NIH/3T3 cells using HMGCR antibody (CAB16876) at dilution of 1:100. Blue: DAPI for nuclear staining.

Synonyms:

HMGCR, LDLCQ3, HMGCR

Background:

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.
UniProt Protein Details:	Protein type:EC 1.1.1.34; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Endoplasmic reticulum; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 5q13.3-q14 Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; protein homodimerization activity; hydroxymethylglutaryl-CoA reductase activity; coenzyme binding Biological Process: negative regulation of MAP kinase activity; isoprenoid biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of skeletal muscle development; cellular lipid metabolic process; cholesterol biosynthetic process; positive regulation of stress-activated MAPK cascade; coenzyme A metabolic process; myoblast differentiation; ubiquinone metabolic process; response to ethanol; negative regulation of vasodilation; visual learning; protein tetramerization; response to nutrient; aging
NCBI Summary:	HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	P04035
NCBI GenInfo Identifier:	123343
NCBI Gene ID:	3156
NCBI Accession:	P04035.1
UniProt Secondary Accession:	P04035,Q8N190, B7Z3Y9,
UniProt Related Accession:	P04035
Molecular Weight:	888
NCBI Full Name:	3-hydroxy-3-methylglutaryl-coenzyme A reductase
NCBI Synonym Full Names:	3-hydroxy-3-methylglutaryl-CoA reductase
NCBI Official Symbol:	HMGCR
NCBI Official Synonym Symbols:	LDLCQ3
NCBI Protein Information:	3-hydroxy-3-methylglutaryl-Coenzyme A reductase; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH)
UniProt Protein Name:	3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProt Gene Name:	HMGCR
UniProt Entry Name:	HMDH_HUMAN

Antibodies	ELISA Kits
Anti-HMGCR Antibody (CAB13314)	Human HMGCR ELISA Kit
Anti-HMGCR Antibody (CAB14741)[KO Validated]	Human 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) ELISA Kit
Anti-HMGCR Antibody (CAB1633)
Anti-HMGCR Antibody (CAB16875)
Anti-HMGCR Antibody (CAB19063)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)