Anti-KPNA4 Antibody (CAB2026)

Antibody Name:	Anti-KPNA4 Antibody
Antibody SKU:	CAB2026
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KPNA4 (NP_002259.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, A-549, SKOV3, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human KPNA4 (NP_002259.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADN EKLD NQRL KNFK NKGR DLET MRRQ RNEV VVEL RKNK RDEH LLKR RNVP HEDI CEDS DIDG DYRV QNTS LEAI VQNA SSDN QGIQ LSAV QAAR KLLS SDRN PPID DLIK SGIL PILV HCLE RDDN PSLQ FEAA WALT NIAS GTSE QTQA VVQS NAVP LFLR LLHS PHQN VCEQ AVWA LGNI IGDG PQCR DYVI SLGV VKPL LSFI SPSI PITF LRNV TWVM VNLC RHKD PPPP METI
Gene ID:	3840
Uniprot:	O00629
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	57kDa
Observed MW:	58kDa

Synonyms:

KPNA4, IPOA3, QIP1, SRP3

Background:

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

UniProt Protein Function:	KPNA4: an alpha importin protein that functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Forms a complex with importin beta-1 subunit.
UniProt Protein Details:	Protein type:Karyopherin; Chaperone; Nuclear import; Adaptor/scaffold Chromosomal Location of Human Ortholog: 3q25.33 Cellular Component: cytosol; nuclear pore; nucleoplasm Molecular Function:nuclear localization sequence binding; protein binding; protein transporter activity Biological Process: modification by virus of host cellular process; NLS-bearing substrate import into nucleus
NCBI Summary:	The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen. [provided by RefSeq, Jul 2008]
UniProt Code:	O00629
NCBI GenInfo Identifier:	2811003
NCBI Gene ID:	3840
NCBI Accession:	O00629.1
UniProt Secondary Accession:	O00629,O00190, A8K4S6, D3DNM2,
UniProt Related Accession:	O00629
Molecular Weight:	57,887 Da
NCBI Full Name:	Importin subunit alpha-3
NCBI Synonym Full Names:	karyopherin subunit alpha 4
NCBI Official Symbol:	KPNA4
NCBI Official Synonym Symbols:	QIP1; SRP3; IPOA3
NCBI Protein Information:	importin subunit alpha-3
UniProt Protein Name:	Importin subunit alpha-3
UniProt Synonym Protein Names:	Importin alpha Q1; Qip1; Karyopherin subunit alpha-4
UniProt Gene Name:	KPNA4
UniProt Entry Name:	IMA3_HUMAN

	Western blot analysis of extracts of various cell lines, using KPNA4 antibody (CAB2026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat heart using KPNA4 Antibody (CAB2026) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using KPNA4 Antibody (CAB2026) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using KPNA4 antibody (CAB2026). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
KPNA4 Antibody (PACO10153)	Human KPNA4 (Karyopherin subunit alpha-4) ELISA Kit (HUFI03671)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)