The KPNA4 Antibody (CAB2026) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been verified for use in Western blot applications. By binding specifically to the KPNA4 protein, this antibody enables accurate detection and analysis in a variety of cell types.KPNA4, also known as Karyopherin alpha 4, plays a crucial role in the transport of molecules into the nucleus, making it essential for various cellular processes such as gene expression and signaling. Dysregulation of KPNA4 has been implicated in diseases like cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KPNA4 Antibody
SKU:
CAB2026
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
QIP1, SRP3, IPOA3, KPNA4
Positive Sample:
A-549
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
58kDa
Observed MW:
58kDa
The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.
Purification Method
Affinity purification
Gene ID
3840
RRID
AB_2764050
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-549 cells, using KPNA4 Rabbit pAb (CAB2026) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using KPNA4 Rabbit pAb (CAB2026) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U2OS cells using KPNA4 Rabbit pAb (CAB2026). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
