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Epigenetics and Nuclear Signaling Antibodies 5 Anti-Ku70 Antibody CAB11223
Key Features

Ku70 Rabbit Monoclonal Antibody (CAB11223)

Datasheet MSDS
SKU CAB11223
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms ML8, KU70, TLAA, CTC75, CTCBF, G22P1, Ku70
Research Use Epigenetics and Nuclear Signaling
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

Ku70 Monoclonal Antibody (CAB11223)

The Ku70 Monoclonal Antibody (CAB11223) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and is highly specific to human samples, making it ideal for use in various research applications, including Western blotting and immunofluorescence.The KU70 protein is a key component of the DNA-dependent protein kinase complex, which is involved in the repair of DNA double-strand breaks. Dysregulation of KU70 has been linked to genetic instability and the development of various diseases, including cancer.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:Ku70 Monoclonal Antibody
SKU:CAB11223
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0551
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:PEQA VDLT LPKV EAMN KRLG SLVD EFKE LVYP PDYN PEGK VTKR KHDN EGSG SKRP KVEY SEEE LKTH ISKG TLGK FTVP MLKE ACRA YGLK SGLK KQEL LEAL TKHF QD
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:3000 - 1:20000
IHC-P1:200 - 1:800
IF/ICC1:100 - 1:500
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:ML8, KU70, TLAA, CTC75, CTCBF, G22P1, Ku70
Positive Sample:A-549
Cellular Localization:Chromosome, Nucleus.
Calculated MW:70kDa
Observed MW:70kDa

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Purification MethodAffinity purification
Gene ID2547
RRIDAB_2861526
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from A549 cells using Ku70 Rabbit mAb (CAB11223) at 1:6000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Ku70 Rabbit mAb (CAB11223) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Ku70 Rabbit mAb (CAB11223) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Product ImageConfocal imaging of NIH/3T3 cells using Ku70 Rabbit mAb (CAB11223, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Product ImageConfocal imaging of C6 cells using Ku70 Rabbit mAb (CAB11223, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
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