Anti-SELL Antibody (CAB1622)

Antibody Name:	Anti-SELL Antibody
Antibody SKU:	CAB1622
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SELL (NP_000646.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Raji, Mouse spleen, Mouse thymus, Rat thymus

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SELL (NP_000646.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGCR RTRE GPSK AMIF PWKC QSTQ RDLW NIFK LWGW TMLC CDFL AHHG TDCW TYHY SEKP MNWQ RARR FCRD NYTD LVAI QNKA EIEY LEKT LPFS RSYY
Gene ID:	6402
Uniprot:	P14151
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	42kDa/43kDa
Observed MW:	74kDa

Synonyms:

SELL, CD62L, LAM1, LECAM1, LEU8, LNHR, LSEL, LYAM1, PLNHR, TQ1

Background:

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	SELL: Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. Belongs to the selectin/LECAM family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1q23-q25 Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane Molecular Function:heparin binding; protein binding; protease binding; cell adhesion molecule binding; glycosphingolipid binding; carbohydrate binding Biological Process: regulation of immune response; blood coagulation; cell adhesion; leukocyte migration; response to ATP Disease: Iga Nephropathy, Susceptibility To, 1
NCBI Summary:	This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]
UniProt Code:	P14151
NCBI GenInfo Identifier:	126178
NCBI Gene ID:	6402
NCBI Accession:	P14151.2
UniProt Secondary Accession:	P14151,P15023, Q9UJ43, B2R6Q8,
UniProt Related Accession:	P14151
Molecular Weight:	43,618 Da
NCBI Full Name:	L-selectin
NCBI Synonym Full Names:	selectin L
NCBI Official Symbol:	SELL
NCBI Official Synonym Symbols:	TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
NCBI Protein Information:	L-selectin; gp90-MEL; pln homing receptor; lymph node homing receptor; lymphocyte adhesion molecule 1; leukocyte surface antigen Leu-8; CD62 antigen-like family member L; leukocyte-endothelial cell adhesion molecule 1
UniProt Protein Name:	L-selectin
UniProt Synonym Protein Names:	CD62 antigen-like family member L; Leukocyte adhesion molecule 1; LAM-1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LECAM1; Lymph node homing receptor; TQ1; gp90-MEL; CD_antigen: CD62L
Protein Family:	L-selectin
UniProt Gene Name:	SELL
UniProt Entry Name:	LYAM1_HUMAN

	Western blot analysis of extracts of various cell lines, using SELL antibody (CAB1622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat testis using SELL antibody (CAB1622) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human tonsil using SELL antibody (CAB1622) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using SELL antibody (CAB1622) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH/3T3 cells using SELL antibody (CAB1622) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Human CD62L Monoclonal Antibody [DREG56](AGEL0222)	Human L-Selectin / CD62L ELISA Kit
Human CD62L Monoclonal Antibody (Biotin Conjugated) [DREG56](AGEL0223)	Human SELL (L-Selectin) CLIA Kit (HUES00536)
Human CD62L Monoclonal Antibody (FITC Conjugated) [DREG56](AGEL0224)	Human SELL (L-Selectin) ELISA Kit (HUES02021)
Human CD62L Monoclonal Antibody (APC Conjugated) [DREG56](AGEL0225)	Human L-selectin (SELL) ELISA Kit
Human CD62L Monoclonal Antibody (Alexa Fluor 488 Conjugated) [DREG56](AGEL0226)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)