LAMB4 Antibody (PACO63339)
- SKU:
- PACO63339
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | LAMB4 Antibody (PACO63339) |
| Antibody SKU: | PACO63339 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Laminin subunit beta-4 protein (1513-1761AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63339 diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO63339 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO63339 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. |
| Synonyms: | Laminin subunit beta-4 (Laminin beta-1-related protein), LAMB4 |
| UniProt Protein Function: | LAMB4: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 7q31.1 Cellular Component: basement membrane Biological Process: cell adhesion |
| UniProt Code: | A4D0S4 |
| NCBI GenInfo Identifier: | 968121869 |
| NCBI Gene ID: | 22798 |
| NCBI Accession: | NP_001304975.1 |
| UniProt Secondary Accession: | A4D0S4,Q86TP7, Q86XN2, Q8NBX5, A5PKU6, B2RTT3, B5MEB9 |
| UniProt Related Accession: | A4D0S4 |
| Molecular Weight: | |
| NCBI Full Name: | laminin subunit beta-4 isoform 1 |
| NCBI Synonym Full Names: | laminin subunit beta 4 |
| NCBI Official Symbol: | LAMB4Â Â |
| NCBI Protein Information: | laminin subunit beta-4 |
| UniProt Protein Name: | Laminin subunit beta-4 |
| UniProt Synonym Protein Names: | Laminin beta-1-related protein |
| Protein Family: | Laminin |
| UniProt Gene Name: | LAMB4Â Â |
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