Anti-LCK Antibody (CAB2177)

Antibody Name:	Anti-LCK Antibody
Antibody SKU:	CAB2177
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LCK (NP_001036236.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, Mouse lung, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LCK (NP_001036236.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGCG CSSH PEDD WMEN IDVC ENCH YPIV PLDG KGTL LIRN GSEV RDPL VTYE GSNP PASP LQDN LVIA LHSY EPSH DGDL GFEK GEQL RILE QSGE WWKA QSLT TGQE GFIP FNFV AKAN SLEP EPWF FKNL SRKD AERQ LLAP GNTH GSFL IRES ESTA GSFS LSVR DFDQ NQGE VVKH YKIR NLDN GGFY ISPR ITFP GLHE LVRH YTNA SDGL CTRL SRPC QTQK PQKP WWED EWEV
Gene ID:	3932
Uniprot:	P06239
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, Lipid-anchor
Calculated MW:	40kDa/58kDa/61kDa
Observed MW:	58KDa

Synonyms:

LCK, IMD22, LSK, YT16, p56lck, pp58lck

Background:

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.

UniProt Protein Function:	Lck: a tyrosine kinase of the Src family that is crucial to antigen-receptor signaling in lymphocytes. plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of Sam68 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Overexpression in mice leads to thymic tumors. Aberrant expression is seen in T cell leukemias and colon cancer. The leukemic translocation t(1;7)(p34;q34) has breakpoints at the T cell receptor gene and close to the Lck promoters, can cause increased Lck expression, and in one case, point mutations. A mutated Lck has also been seen in a cell line. One patient with aberrant Lck splicing suffered from SCID-like T cell deficiency. Inhibitor: BMS-279700. Three alternatively spliced isoforms of the human proteinhave been described.
UniProt Protein Details:	Protein type:Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Oncoprotein; Kinase, protein; TK group; Src family Chromosomal Location of Human Ortholog: 1p34.3 Cellular Component: pericentriolar material; extrinsic to internal side of plasma membrane; endocytic vesicle; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft Molecular Function:protein C-terminus binding; identical protein binding; CD8 receptor binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine phosphatase activity; antigen binding; protein kinase binding; protein phosphatase binding; ATPase binding; protein binding; protein-tyrosine kinase activity; protein complex binding; phosphoinositide 3-kinase binding; SH2 domain binding; glycoprotein binding; CD4 receptor binding; ATP binding Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; protein amino acid phosphorylation; T cell receptor signaling pathway; B cell receptor signaling pathway; positive regulation of T cell receptor signaling pathway; hemopoiesis; positive regulation of gamma-delta T cell differentiation; aging; T cell differentiation; caspase activation; response to drug; regulation of lymphocyte activation; epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cellular zinc ion homeostasis; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat5 protein; response to hydrogen peroxide; dephosphorylation; response to mechanical stimulus; response to zinc ion; release of sequestered calcium ion into cytosol; T cell costimulation; innate immune response; positive regulation of T cell activation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway Disease: Immunodeficiency 22
NCBI Summary:	This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P06239
NCBI GenInfo Identifier:	125474
NCBI Gene ID:	3932
NCBI Accession:	P06239.6
UniProt Secondary Accession:	P06239,P07100, Q12850, Q13152, Q5TDH8, Q5TDH9, Q7RTZ3 Q96DW4, Q9NYT8, D3DPP8,
UniProt Related Accession:	P06239
Molecular Weight:	Calculated Molecular Weight: 40kDa/58kDa/61kDaObserved Molecular Weight: 55kDa
NCBI Full Name:	Tyrosine-protein kinase Lck
NCBI Synonym Full Names:	LCK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol:	LCK
NCBI Official Synonym Symbols:	LSK; YT16; IMD22; p56lck; pp58lck
NCBI Protein Information:	tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte-specific protein tyrosine kinase; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck
UniProt Protein Name:	Tyrosine-protein kinase Lck
UniProt Synonym Protein Names:	Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Protein Family:	Tyrosine-protein kinase
UniProt Gene Name:	LCK
UniProt Entry Name:	LCK_HUMAN

	Western blot analysis of extracts of Mouse thymus, using LCK antibody (CAB2177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using LCK antibody (CAB2177) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spleen using LCK Rabbit pAb (CAB2177) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using LCK Rabbit pAb (CAB2177) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using LCK Rabbit pAb (CAB2177) at dilution of 1:100 (40x lens).