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Anti-LCN2 Antibody (CAB2092)

SKU:
CAB2092
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
  • Cell Death Antibodies 1 Anti-LCN2 Antibody CAB2092
  • Cell Death Antibodies 1 Anti-LCN2 Antibody CAB2092
€139 - €419
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Description

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Antibody Name:Anti-LCN2 Antibody
Antibody SKU:CAB2092
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-198 of human LCN2 (NP_005555.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:DU145, HT-29, BxPC-3, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-198 of human LCN2 (NP_005555.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QDST SDLI PAPP LSKV PLQQ NFQD NQFQ GKWY VVGL AGNA ILRE DKDP QKMY ATIY ELKE DKSY NVTS VLFR KKKC DYWI RTFV PGCQ PGEF TLGN IKSY PGLT SYLV RVVS TNYN QHAM VFFK KVSQ NREY FKIT LYGR TKEL TSEL KENF IRFS KSLG LPEN HIVF PVPI DQCI DG
Gene ID:3934
Uniprot:P80188
Cellular Location:Secreted
Calculated MW:22kDa
Observed MW:22kDa
Synonyms:LCN2, 24p3, MSFI, NGAL, p25
Background:This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice.
UniProt Protein Function:LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space; extracellular region; cytosol

Molecular Function:protein homodimerization activity; protease binding; transporter activity; iron ion binding

Biological Process: response to drug; cellular response to nutrient levels; cellular iron ion homeostasis; response to virus; siderophore transport; positive regulation of cell projection organization and biogenesis; innate immune response; ion transport; response to herbicide; transmembrane transport

UniProt Code:P80188
NCBI GenInfo Identifier:1171700
NCBI Gene ID:3934
NCBI Accession:P80188.2
UniProt Secondary Accession:P80188,P30150, Q5SYV9, Q5SYW0, Q6FGL5, Q92683, A6NII8 B4DWV4, B7ZAA2,
UniProt Related Accession:P80188
Molecular Weight:
NCBI Full Name:Neutrophil gelatinase-associated lipocalin
NCBI Synonym Full Names:lipocalin 2
NCBI Official Symbol:LCN2  
NCBI Official Synonym Symbols:24p3; MSFI; NGAL  
NCBI Protein Information:neutrophil gelatinase-associated lipocalin; p25; lipocalin-2; oncogene 24p3; siderocalin LCN2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9
UniProt Protein Name:Neutrophil gelatinase-associated lipocalin
UniProt Synonym Protein Names:25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25
Protein Family:Neutrophil gelatinase-associated lipocalin
UniProt Gene Name:LCN2  
UniProt Entry Name:NGAL_HUMAN
Anti-LCN2 Antibody (CAB2092)Western blot analysis of extracts of various cell lines, using LCN2 antibody (CAB2092) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-LCN2 Antibody (CAB2092)Immunofluorescence analysis of mouse kidney using LCN2 antibody (CAB2092) at dilution of 1:100. Blue: DAPI for nuclear staining.

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