Anti-LMAN2 Antibody (CAB15394)

Antibody Name:	Anti-LMAN2 Antibody
Antibody SKU:	CAB15394
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 190-320 of human LMAN2 (NP_006807.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse
Positive Samples:	Mouse brain, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 190-320 of human LMAN2 (NP_006807.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HSKD GRWT ELAG CTAD FRNR DHDT FLAV RYSR GRLT VMTD LEDK NEWK NCID ITGV RLPT GYYF GASA GTGD LSDN HDII SMKL FQLM VEHT PDEE SIDW TKIE PSVN FLKS PKDN VDDP TGNF RSGP LTG
Gene ID:	10960
Uniprot:	Q12907
Cellular Location:	Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Golgi apparatus membrane, Single-pass membrane protein, Single-pass type I membrane protein
Calculated MW:	40kDa
Observed MW:	39kDa

Synonyms:	LMAN2, C5orf8, GP36B, VIP36, lectin
Background:	This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quality control.

UniProt Protein Function:	LMAN2: Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 5q35.3 Cellular Component: cell surface; ER-Golgi intermediate compartment; extracellular space; Golgi apparatus; integral to plasma membrane Molecular Function:carbohydrate binding; glycoprotein binding; heat shock protein binding; mannose binding Biological Process: positive regulation of phagocytosis; retrograde vesicle-mediated transport, Golgi to ER
NCBI Summary:	This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quality control. [provided by RefSeq, Oct 2008]
UniProt Code:	Q12907
NCBI GenInfo Identifier:	21264108
NCBI Gene ID:	10960
NCBI Accession:	Q12907.1
UniProt Secondary Accession:	Q12907,Q53HH1,
UniProt Related Accession:	Q12907
Molecular Weight:	40,229 Da
NCBI Full Name:	Vesicular integral-membrane protein VIP36
NCBI Synonym Full Names:	lectin, mannose binding 2
NCBI Official Symbol:	LMAN2
NCBI Official Synonym Symbols:	GP36B; VIP36; C5orf8
NCBI Protein Information:	vesicular integral-membrane protein VIP36
UniProt Protein Name:	Vesicular integral-membrane protein VIP36
UniProt Synonym Protein Names:	Glycoprotein GP36b; Lectin mannose-binding 2; Vesicular integral-membrane protein 36; VIP36
Protein Family:	Vesicular integral-membrane protein
UniProt Gene Name:	LMAN2
UniProt Entry Name:	LMAN2_HUMAN

	Western blot analysis of extracts of various cell lines, using LMAN2 antibody (CAB15394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using LMAN2 antibody (CAB15394) at dilution of 1:100. Blue: DAPI for nuclear staining.

ELISA Kits

Human LMAN2 / VIP36 ELISA Kit

Human Vesicular integral-membrane protein VIP36 (LMAN2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)