The LMAN2 Antibody (CAB15394) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for the human LMAN2 protein and has been validated for use in Western blot applications. By binding to LMAN2, this antibody allows for the detection and analysis of this important protein in various cell types, making it an excellent choice for research in cell biology and molecular biology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
LMAN2 Antibody
SKU:
CAB15394
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GP36B, VIP36, C5orf8, LMAN2
Positive Sample:
Mouse brain, Mouse heart, Mouse kidney
Cellular Localization:
Endoplasmic Reticulum Membrane, Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Golgi Apparatus Membrane, Single-Pass Membrane Protein, Single-Pass Type I Membrane Protein.
Calculated MW:
40kDa
Observed MW:
39kDa
This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quality control.
Purification Method
Affinity purification
Gene ID
10960
RRID
AB_2762301
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using LMAN2 Rabbit pAb (CAB15394) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of L929 cells using LMAN2 Rabbit pAb (CAB15394) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
