Anti-LNPEP Antibody (CAB11959)

Antibody Name:	Anti-LNPEP Antibody
Antibody SKU:	CAB11959
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human LNPEP (NP_005566.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HT-29, U-87MG, HeLa, Jurkat, Mouse brain, Mouse heart, Mouse liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human LNPEP (NP_005566.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEPF TNDR LQLP RNMI ENSM FEEE PDVV DLAK EPCL HPLE PDEV EYEP RGSR LLVR GLGE HEME EDEE DYES SAKL LGMS FMNR SSGL RNSA TGYR QSPD GACS VPSA RT
Gene ID:	4012
Uniprot:	Q9UIQ6
Cellular Location:	Cell membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	115kDa/117kDa
Observed MW:	160kDa

Synonyms:

LNPEP, CAP, IRAP, P-LAP, PLAP

Background:

This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	IRAP: a leucyl/cystinyl aminopeptidase. A type II membrane protein and secreted. Resides in intracellular vesicles together with GLUT4 and can then translocate to the cell surface in response to insulin and/or oxytocin. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:EC 3.4.11.3; Membrane protein, integral; Protease Chromosomal Location of Human Ortholog: 5q15 Cellular Component: cytoplasmic vesicle membrane; cytosol; integral to plasma membrane; intracellular; lysosomal membrane; membrane; plasma membrane Molecular Function:aminopeptidase activity; metallopeptidase activity; peptide binding; protein binding; zinc ion binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cell-cell signaling; female pregnancy; peptide catabolic process; protein polyubiquitination; regulation of blood pressure; signal transduction
NCBI Summary:	This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UIQ6
NCBI GenInfo Identifier:	145559489
NCBI Gene ID:	4012
NCBI Accession:	Q9UIQ6.3
UniProt Secondary Accession:	Q9UIQ6,O00769, Q15145, Q59H76, Q9TNQ2, Q9TNQ3, Q9UIQ7
UniProt Related Accession:	Q9UIQ6
Molecular Weight:	117kDa
NCBI Full Name:	Leucyl-cystinyl aminopeptidase
NCBI Synonym Full Names:	leucyl and cystinyl aminopeptidase
NCBI Official Symbol:	LNPEP
NCBI Official Synonym Symbols:	CAP; IRAP; PLAP; P-LAP
NCBI Protein Information:	leucyl-cystinyl aminopeptidase
UniProt Protein Name:	Leucyl-cystinyl aminopeptidase
UniProt Synonym Protein Names:	Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; IRAP
Protein Family:	Leucyl-cystinyl aminopeptidase
UniProt Gene Name:	LNPEP

	Western blot analysis of extracts of various cell lines, using LNPEP antibody (CAB11959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat liver using LNPEP Rabbit pAb (CAB11959) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using LNPEP Rabbit pAb (CAB11959) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using LNPEP Rabbit pAb (CAB11959) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
LNPEP Antibody (PACO10274)	Human Leucyl-cystinyl aminopeptidase / LNPEP ELISA Kit
	Human Leucyl-cystinyl aminopeptidase (LNPEP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)