Anti-LRAT Antibody (CAB6647)

Antibody Name:	Anti-LRAT Antibody
Antibody SKU:	CAB6647
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human LRAT (NP_004735.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human LRAT (NP_004735.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKNP MLEV VSLL LEKL LLIS NFTL FSSG AAGE DKGR NSFY ETSS FHRG DVLE VPRT HLTH YGIY LGDN RVAH MMPD ILLA LTDD MGRT QKVV SNKR LILG VIVK VASI RVDT VEDF AYGA NILV NHLD ESLQ KKAL LNEE VARR AEKL LGFT PYSL LWNN CEHF VTYC RYGT PISP QSDK FCET VKII IRDQ RS
Gene ID:	9227
Uniprot:	O95237
Cellular Location:	Cytoplasm, Endoplasmic reticulum membrane, Endosome, Rough endoplasmic reticulum, Single-pass membrane protein, multivesicular body, perinuclear region
Calculated MW:	25kDa
Observed MW:	24kDa

Synonyms:

LRAT, LCA14

Background:

The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	LRAT: Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. Retinyl esters are storage forms of vitamin A. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments. Defects in LRAT are a cause of Leber congenital amaurosis type 14 (LCA14). It is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the H-rev107 family.
UniProt Protein Details:	Protein type:EC 2.3.1.135; Transferase; Cofactor and Vitamin Metabolism - retinol; Membrane protein, integral Chromosomal Location of Human Ortholog: 4q32.1 Cellular Component: multivesicular body; endoplasmic reticulum membrane; rough endoplasmic reticulum; perinuclear region of cytoplasm; integral to membrane Molecular Function:retinoic acid binding; transferase activity, transferring acyl groups; retinol binding; phosphatidylcholine-retinol O-acyltransferase activity Biological Process: positive regulation of lipid transport; phototransduction, visible light; visual perception; retinol metabolic process; retinoic acid metabolic process; retinoid metabolic process; vitamin A metabolic process Disease: Leber Congenital Amaurosis 14; Retinitis Pigmentosa
NCBI Summary:	The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
UniProt Code:	O95237
NCBI GenInfo Identifier:	46576867
NCBI Gene ID:	9227
NCBI Accession:	O95237.2
UniProt Secondary Accession:	O95237,Q8N716, A8K983,
UniProt Related Accession:	O95237
Molecular Weight:	25,703 Da
NCBI Full Name:	Lecithin retinol acyltransferase
NCBI Synonym Full Names:	lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
NCBI Official Symbol:	LRAT
NCBI Official Synonym Symbols:	LCA14
NCBI Protein Information:	lecithin retinol acyltransferase
UniProt Protein Name:	Lecithin retinol acyltransferase
UniProt Synonym Protein Names:	Phosphatidylcholine--retinol O-acyltransferase
Protein Family:	Lecithin retinol acyltransferase
UniProt Gene Name:	LRAT
UniProt Entry Name:	LRAT_HUMAN

	Western blot analysis of extracts of rat testis, using Lrat antibody (CAB6647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using LRAT antibody (CAB6647). Blue: DAPI for nuclear staining.

Antibodies

Anti-LRAT Antibody (CAB13175)

Anti-LRAT Antibody (CAB13256)

LRAT Antibody (PACO01029)

LRAT Antibody (PACO10284)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)