Anti-LRP2 Antibody (CAB3612)

Antibody Name:	Anti-LRP2 Antibody
Antibody SKU:	CAB3612
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human LRP2 (NP_004516.2).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:20 - 1:100
Reactivity:	Mouse, Rat
Positive Samples:	Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human LRP2 (NP_004516.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FHYR RTGS LLPA LPKL PSLS SLVK PSEN GNGV TFRS GADL NMDI GVSG FGPE TAID RSMA MSED FVME MGKQ PIIF ENPM YSAR DSAV KVVQ PIQV TVSE NVDN KNYG SPIN PSEI VPET NPTS PAAD GTQV TKWN LFKR KSKQ TTNF ENPI YAQM ENEQ KESV AATP PPSP SLPA KPKP PSRR DPTP TYSA TEDT FKDT ANLV KEDS EV
Gene ID:	4036
Uniprot:	P98164
Cellular Location:	Membrane, Single-pass type I membrane protein, coated pit
Calculated MW:	521kDa
Observed MW:	522kDa

Synonyms:

LRP2, DBS, GP330

Background:

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

UniProt Protein Function:	LRP2: Acts together with cubilin to mediate HDL endocytosis. May participate in regulation of parathyroid- hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS); also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. Belongs to the LDLR family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc. Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: Golgi apparatus; endoplasmic reticulum; brush border membrane; endocytic vesicle; lysosomal membrane; lysosome; apical plasma membrane; integral to membrane; plasma membrane; coated pit; endosome; receptor complex Molecular Function:protein binding; calcium ion binding; SH3 domain binding Biological Process: steroid metabolic process; receptor-mediated endocytosis; cell proliferation; phototransduction, visible light; forebrain development; protein amino acid glycosylation; endocytosis; lipid metabolic process; retinoid metabolic process; vitamin D metabolic process Disease: Donnai-barrow Syndrome
NCBI Summary:	The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]
UniProt Code:	P98164
NCBI GenInfo Identifier:	160332309
NCBI Gene ID:	4036
NCBI Accession:	P98164.3
UniProt Related Accession:	P98164
Molecular Weight:
NCBI Full Name:	Low-density lipoprotein receptor-related protein 2
NCBI Synonym Full Names:	LDL receptor related protein 2
NCBI Official Symbol:	LRP2
NCBI Official Synonym Symbols:	DBS; GP330; LRP-2
NCBI Protein Information:	low-density lipoprotein receptor-related protein 2
UniProt Protein Name:	Low-density lipoprotein receptor-related protein 2
UniProt Synonym Protein Names:	Glycoprotein 330; gp330; Megalin
Protein Family:	Low-density lipoprotein receptor-related protein
UniProt Gene Name:	LRP2
UniProt Entry Name:	LRP2_HUMAN

	Western blot analysis of extracts of mouse lung, using LRP2 antibody (CAB3612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded rat kidney using LRP2 antibody (CAB3612) at dilution of 1:100 (40x lens).

ELISA Kits

Human LRP2 ELISA Kit

Human Low-density lipoprotein receptor-related protein 2 (LRP2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)