Anti-SSB Antibody (CAB0630)

Antibody Name:	Anti-SSB Antibody
Antibody SKU:	CAB0630
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 166-408 of human SSB (NP_003133.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human
Positive Samples:	Jurkat, LO2, HepG2, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 166-408 of human SSB (NP_003133.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KFVE TPGQ KYKE TDLL ILFK DDYF AKKN EERK QNKV EAKL RAKQ EQEA KQKL EEDA EMKS LEEK IGCL LKFS GDLD DQTC REDL HILF SNHG EIKW IDFV RGAK EGII LFKE KAKE ALGK AKDA NNGN LQLR NKEV TWEV LEGE VEKE ALKK IIED QQES LNKW KSKG RRFK GKGK GNKA AQPG SGKG KVQF QGKK TKFA SDDE HDEH DENG ATGP VKRA REET DKEE PASK QQKT ENGA GDQ
Gene ID:	6741
Uniprot:	P05455
Cellular Location:	Nucleus
Calculated MW:	46kDa
Observed MW:	47kDa

Synonyms:

SSB, LARP3, La, La/SSB

Background:

The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.

UniProt Protein Function:	SSB: a nuclear protein that plays a role in the transcription of RNA polymerase III. It is most probably a transcription termination factor. Binds to the 3' termini of virtually all nascent polymerase III transcripts. It is associated with precursor forms of RNA polymerase III transcripts including tRNA and 4.5S, 5s, 7s, and 7-2 RNAs.
UniProt Protein Details:	Protein type:Transcription initiation complex Chromosomal Location of Human Ortholog: 2q31.1 Cellular Component: nuclear chromosome, telomeric region; ribonucleoprotein complex Molecular Function:mRNA binding; nucleotide binding; protein binding; tRNA binding Biological Process: histone mRNA metabolic process; tRNA modification
NCBI Summary:	The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]
UniProt Code:	P05455
NCBI GenInfo Identifier:	125985
NCBI Gene ID:	6741
NCBI Accession:	P05455.2
UniProt Secondary Accession:	P05455,Q15367, Q53XJ4,
UniProt Related Accession:	P05455
Molecular Weight:	46,837 Da
NCBI Full Name:	Lupus La protein
NCBI Synonym Full Names:	Sjogren syndrome antigen B
NCBI Official Symbol:	SSB
NCBI Official Synonym Symbols:	La; LARP3; La/SSB
NCBI Protein Information:	lupus La protein
UniProt Protein Name:	Lupus La protein
UniProt Synonym Protein Names:	La autoantigen; La ribonucleoprotein; Sjoegren syndrome type B antigen; SS-B
Protein Family:	Lupus La protein
UniProt Gene Name:	SSB
UniProt Entry Name:	LA_HUMAN

	Western blot analysis of extracts of various cell lines, using SSB antibody (CAB0630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human prostate cancer using SSB antibody (CAB0630) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using SSB antibody (CAB0630) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using SSB antibody (CAB0630) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug SSB antibody (CAB0630). Western blot was performed from the immunoprecipitate using SSB antibody (CAB0630) at a dilition of 1:1000.

Antibodies	ELISA Kits
Phospho-SSB (S366) Antibody (PACO05130)	Human SSB(Lupus La protein) ELISA Kit
SSB Antibody (PACO06822)	Human Lupus La protein (SSB) ELISA Kit
SSB Antibody (PACO12472)	SSB Colorimetric Cell-Based ELISA Kit
	SSB (Phospho-Ser366) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)