The MAF1 Antibody (CAB15531) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples. Validated for use in Western blot applications, it specifically binds to the MAF1 protein, allowing for accurate detection and analysis in various cell types.MAF1 is involved in controlling the expression of genes responsible for the production of transfer RNA and ribosomal RNA, making it essential for the regulation of cellular growth and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MAF1 Antibody
SKU:
CAB15531
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MAF1, MAF1 homolog
Positive Sample:
A-549, HeLa, Mouse thymus
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
29kDa
Observed MW:
37kDa
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.
Purification Method
Affinity purification
Gene ID
84232
RRID
AB_2762932
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MAF1 Rabbit pAb (CAB15531) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
