The MAFA Polyclonal Antibody (CAB18662) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific and sensitive, making it ideal for use in various experimental techniques such as Western blotting and immunofluorescence.MAFA is a transcription factor that plays a crucial role in the maintenance of beta cell identity and function, making it a promising target for the development of novel treatments for diabetes and other metabolic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
MAFA Polyclonal Antibody
SKU:
CAB18662
Size:
20μL, 100μL
Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
INSDM, hMafA, RIPE3b1, MAFA
Positive Sample:
Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
37kDa
Observed MW:
37kDa
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).
Purification Method
Affinity purification
Gene ID
389692
RRID
AB_2862399
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain, using MAFA Rabbit pAb (CAB18662) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
