Anti-MAPK7 Antibody (CAB2111)

Antibody Name:	Anti-MAPK7 Antibody
Antibody SKU:	CAB2111
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human MAPK7 (NP_002740.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, RD, C6, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human MAPK7 (NP_002740.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEP LKEE DGED GSAE PPGP VKAE PAHT AASV AAKN LALL KARS FDVT FDVG DEYE IIET IGNG AYGV VSSA RRRL TGQQ VAIK KIPN AFDV VTNA KRTL RELK ILKH FKHD NIIA IKDI LRPT VPYG EFKS VYVV LDLM ESDL HQII HSSQ PLTL EHVR YFLY QLLR GLKY MHSA QVIH RDLK PSNL LVNE NCEL KIGD FGMA RGLC TSPA EHQY FMTE YVAT RWYR APEL MLSL HEYT QAID LWSV GCIF GEML ARRQ LFPG KNYV HQLQ LIMM VLGT PSPA VIQA VGAE RVRA YIQS LPPR QPVP WE
Gene ID:	5598
Uniprot:	Q13164
Cellular Location:	Cytoplasm, Nucleus, PML body
Calculated MW:	50kDa/59kDa/73kDa/88kDa
Observed MW:	115KDa

Synonyms:

MAPK7, BMK1, ERK4, ERK5, PRKM7

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.

UniProt Protein Function:	ERK5: a protein kinase of the MAPK family. Phosphorylated and activated by MEK5. Involved in the signaling processes downstream of various receptors including receptor tyrosine kinases and G protein-coupled receptors. Translocates to cell nucleus in response to extracelluar signals, where it regulates gene expression by phosphorylating various transcription factors. Mouse knockout shows a role in development and integrity of blood vessels. May block apoptosis in endothelial cells. Required for transduction of EGF growth signal, constitutively activated in ErbB2-overexpressing breast cancer, and downstream of activated Mek5 in metastatic prostate cancerFour alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: nucleoplasm; PML body; cytoplasm; nucleus; cytosol Molecular Function:MAP kinase activity; protein binding; mitogen-activated protein kinase binding; ATP binding Biological Process: nerve growth factor receptor signaling pathway; negative regulation of cAMP catabolic process; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; signal transduction; positive regulation of protein metabolic process; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; cAMP-mediated signaling; cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of heterotypic cell-cell adhesion; MyD88-independent toll-like receptor signaling pathway; cell cycle; negative regulation of NFAT protein import into nucleus; regulation of angiogenesis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of cyclic-nucleotide phosphodiesterase activity
NCBI Summary:	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13164
NCBI GenInfo Identifier:	205371766
NCBI Gene ID:	5598
NCBI Accession:	Q13164.2
UniProt Secondary Accession:	Q13164,Q16634, Q59F50, Q6QLU7, Q7L4P4, Q969G1, Q96G51
UniProt Related Accession:	Q13164
Molecular Weight:	50,152 Da
NCBI Full Name:	Mitogen-activated protein kinase 7
NCBI Synonym Full Names:	mitogen-activated protein kinase 7
NCBI Official Symbol:	MAPK7
NCBI Official Synonym Symbols:	BMK1; ERK4; ERK5; PRKM7
NCBI Protein Information:	mitogen-activated protein kinase 7; BMK-1; BMK1 kinase; ERK-5; MAP kinase 7; MAPK 7; big MAP kinase 1; extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5
UniProt Protein Name:	Mitogen-activated protein kinase 7
UniProt Synonym Protein Names:	Big MAP kinase 1; BMK-1; Extracellular signal-regulated kinase 5; ERK-5
UniProt Gene Name:	MAPK7
UniProt Entry Name:	MK07_HUMAN

	Western blot analysis of extracts of various cell lines, using MAPK7 antibody (CAB2111) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using MAPK7 Rabbit pAb (CAB2111) at dilution of 1:50 (40x lens).
	Immunohistochemistry of paraffin-embedded rat liver using MAPK7 Rabbit pAb (CAB2111) at dilution of 1:50 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using MAPK7 antibody (CAB2111). Blue: DAPI for nuclear staining.

Antibodies

Anti-ERK5 Antibody (CAB3948)

MAPK7 Antibody (PACO07005)

MAPK7 Antibody (PACO07006)

MAPK7 Antibody (PACO10381)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)