The MARCH8 Antibody (CAB12962) is a high-quality antibody developed for reliable detection and analysis of target proteins. March8 is a critical regulator of protein turnover and degradation within cells. The antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding to the March8 protein, this antibody enables the detection and analysis of March8 in various cell types, making it an ideal tool for studies in cell biology and protein degradation pathways.March8 plays a crucial role in maintaining cellular homeostasis by targeting specific proteins for degradation, thus regulating key cellular processes such as signal transduction, cell cycle progression, and apoptosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
MARCH8 Antibody
SKU:
CAB12962
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).
Purification Method
Affinity purification
Gene ID
220972
RRID
AB_2759809
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MARCH8 Rabbit pAb (CAB12962) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
