Description
MED1 Colorimetric Cell-Based ELISA
The MED1 Colorimetric Cell-Based ELISA Kit is specifically designed for the precise measurement of MED1 levels in cell lysates and tissue samples. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.MED1 is a key mediator of gene transcription and cell signaling pathways, playing a crucial role in regulating cell growth, differentiation, and survival.
Dysregulation of MED1 has been implicated in various diseases, including cancer, diabetes, and inflammatory disorders, making it a valuable target for therapeutic interventions and biomarker studies.With its user-friendly protocol and reliable performance, the MED1 Colorimetric Cell-Based ELISA Kit is an indispensable tool for researchers looking to investigate the role of MED1 in disease pathogenesis and therapeutic development.
| Product Name: | MED1 Colorimetric Cell-Based ELISA |
| Product Code: | CBCAB01112 |
| ELISA Type: | Cell-Based |
| Target: | MED1 |
| Reactivity: | Human, Mouse |
| Dynamic Range: | > 5000 Cells |
| Detection Method: | Colorimetric 450 nmStorage/Stability:4°C/6 Months |
| Format: | 96-Well Microplate |
The MED1 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect MED1 protein expression profile in cells. The kit can be used for measuring the relative amounts of MED1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on MED1.
Qualitative determination of MED1 concentration is achieved by an indirect ELISA format. In essence, MED1 is captured by MED1-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:
| 1. | A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. |
| 2. | Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. |
| Database Information: | Gene ID: 5469, UniProt ID: Q15648, OMIM: 604311, Unigene: Hs.643754 |
| Gene Symbol: | MED1 |
| Sub Type: | None |
| UniProt Protein Function: | MED1: a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. Also is a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. May regulates p53-dependent apoptosis. Is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. Two splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 17q12 Cellular Component: chromatin; membrane; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex Molecular Function:chromatin binding; chromatin DNA binding; estrogen receptor binding; LBD domain binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; peroxisome proliferator activated receptor binding; protein binding; receptor activity; retinoic acid receptor binding; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription cofactor activity; transcription factor binding; vitamin D receptor binding Biological Process: androgen biosynthetic process; androgen receptor signaling pathway; angiogenesis; brain development; cell morphogenesis; cellular lipid metabolic process; embryonic heart tube development; embryonic hemopoiesis; embryonic hindlimb morphogenesis; embryonic placenta development; enucleate erythrocyte development; erythrocyte development; fat cell differentiation; gene expression; keratinocyte differentiation; lactation; lens development in camera-type eye; liver development; monocyte differentiation; mRNA transcription from RNA polymerase II promoter; negative regulation of apoptosis; negative regulation of neuron differentiation; negative regulation of transcription from RNA polymerase II promoter; organ regeneration; positive regulation of erythrocyte differentiation; positive regulation of estrogen receptor signaling pathway; positive regulation of keratinocyte differentiation; positive regulation of mammary gland epithelial cell proliferation; positive regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein ubiquitination; regulation of cell cycle; regulation of transcription from RNA polymerase I promoter; steroid hormone receptor signaling pathway; thyroid hormone generation; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15648 |
| NCBI GenInfo Identifier: | 158518535 |
| NCBI Gene ID: | 5469 |
| NCBI Accession: | Q15648.4 |
| UniProt Secondary Accession: | Q15648,O43810, O75447, Q6P9H7, Q6PK58, Q9HD39, A2RRQ6 |
| UniProt Related Accession: | Q15648 |
| Molecular Weight: | 61,563 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 1 |
| NCBI Synonym Full Names: | mediator complex subunit 1 |
| NCBI Official Symbol: | MED1 |
| NCBI Official Synonym Symbols: | PBP; CRSP1; RB18A; TRIP2; PPARBP; CRSP200; DRIP205; DRIP230; PPARGBP; TRAP220 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 1 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 1 |
| UniProt Synonym Protein Names: | Activator-recruited cofactor 205 kDa component; ARC205; Mediator complex subunit 1; Peroxisome proliferator-activated receptor-binding protein; PBP; PPAR-binding protein; Thyroid hormone receptor-associated protein complex 220 kDa component; Trap220; Thyroid receptor-interacting protein 2; TR-interacting protein 2; TRIP-2; Vitamin D receptor-interacting protein complex component DRIP205; p53 regulatory protein RB18A |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED1 |
| UniProt Entry Name: | MED1_HUMAN |
| Component | Quantity |
| 96-Well Cell Culture Clear-Bottom Microplate | 2 plates |
| 10X TBS | 24 mL |
| Quenching Buffer | 24 mL |
| Blocking Buffer | 50 mL |
| 15X Wash Buffer | 50 mL |
| Primary Antibody Diluent | 12 mL |
| 100x Anti-Phospho Target Antibody | 60 µL |
| 100x Anti-Target Antibody | 60 µL |
| Anti-GAPDH Antibody | 60 µL |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12 mL |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12 mL |
| SDS Solution | 12 mL |
| Stop Solution | 24 mL |
| Ready-to-Use Substrate | 12 mL |
| Crystal Violet Solution | 12 mL |
| Adhesive Plate Seals | 2 seals |
The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing ≥1 ml
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
- Orbital shaker (optional)
- Deionized or sterile water
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1. | Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells. |
| 2. | Incubate the cells for overnight at 37°C, 5% CO2. |
| 3. | Treat the cells as desired. |
| 4. | Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice. |
| 5. | Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells. |
| 6. | Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week. |
| 7. | Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature. |
| 8. | Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time. |
| 9. | Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature. |
| 10. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 11. | Add 50 µL of 1x primary antibodies (Anti-MED1 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature. |
| 12. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 13. | Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature. |
| 14. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 15. | Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark. |
| 16. | Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader. |
(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)
