The MED20 Antibody (CAB15757) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the MED20 protein, this antibody allows for easy detection and analysis in a variety of cell types, making it an ideal tool for research in the fields of molecular biology and gene regulation.MED20 is a subunit of the mediator complex, which functions as a transcriptional coactivator to regulate gene expression. Its involvement in gene transcription makes it a crucial target for studying various cellular processes, including cell growth, differentiation, and response to stimuli.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MED20 Antibody
SKU:
CAB15757
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SRB2, TRFP, PRO0213, MED20
Positive Sample:
DU145, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
23kDa
Observed MW:
24kDa
This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified.
Purification Method
Affinity purification
Gene ID
9477
RRID
AB_2763174
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MED20 Rabbit pAb (CAB15757) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
