MEL (Melamine) ELISA Kit (FSES0009)



Technical Manual

MEL (Melamine) ELISA Kit

Kit Name: MEL (Melamine) ELISA Kit
Kit Size: 96 Assays
Test Principle: This kit uses Competitive-ELISA as the method. It can detect Melamine (MEL) in samples, such as milk, muscle, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, MEL in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-MEL antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of MEL. The concentration of MEL in the samples can be calculated by comparing the OD of the samples to the standard curve.
Sensitivity: 2 ppb (ng/mL)
Reaction Mode: 25°C, 30 min~15 min
Detection Limit: Milk powder---40 ppb; Milk---54 ppb; Milk, Milk powder (method 2) ---2 ppb; Feed---200 ppb Muscle, Liver ---4 ppb; Eggs---40 ppb; Serum---8 ppb
Cross Reactivity: Melamine ---100%, Cyanuric Acid---60%, s-Triazine <1%
Sample Recovery Rate: ---90±25%
Kits Components:
Item Specifications
ELISA Microtiter plate 96 wells
Standard Liquid 1 mL each (0 ppb,2 ppb,6 ppb,18 ppb,54 ppb, 162 ppb)
HRP Conjugate 5.5 mL
Antibody Working Solution 5.5 mL
Substrate Reagent A 6 mL
Substrate Reagent B 6 mL
Stop Solution 6 mL
20 x Concentrated Wash Buffer 40 mL
2 x Reconstitution Buffer 50 mL
Plate Sealer 3 pieces
Sealed Bag 1 piece
Manual 1 copy
Instruments: Microplate reader, Printer, Homogenizer, Nitrogen evaporators, Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).
Micropipette: Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).
Reagents: N-hexane, Acetonitrile, NaOH, Concentrated HCl, Methanol.
Storage And Expiry Date: Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.
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