The Merlin Polyclonal Antibody (CAB22558) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly reactive with human samples and is validated for use in Western blot applications. By binding to the CD300A protein, researchers can accurately detect and analyze this molecule in various cell types, making it ideal for studies in immunology and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Merlin Polyclonal Antibody
SKU:
CAB22558
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ACN, SCH, BANF, merlin-1, Merlin
Positive Sample:
Jurkat, MCF7, HeLa
Cellular Localization:
Cortical Actin Cytoskeleton, Cytoplasm, Cytoskeleton, Cytosol, Early Endosome, Filopodium Membrane, Nucleolus, Nucleus, Perinuclear Region Of Cytoplasm, Plasma Membrane, Ruffle Membrane.
Calculated MW:
70kDa
Observed MW:
75kDa
This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.
Purification Method
Affinity purification
Gene ID
4771
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Merlin Rabbit pAb (CAB22558) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
