Western blot analysis of various lysates, using MERS-CoVNucleoprotein antibody (CAB21179) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The MERS-CoV Nucleoprotein Monoclonal Antibody (CAB21179) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody is specifically designed to target and detect the MERS-CoV nucleoprotein, allowing for precise analysis and evaluation in various research applications.Raised in mice, this antibody is highly specific and reactive with samples from MERS-CoV-infected individuals, making it a reliable tool for detecting and studying the virus. Its validation for use in techniques such as Western blot and immunofluorescence enables researchers to accurately assess the presence and localization of the MERS-CoV nucleoprotein in cell cultures and tissue samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against MERS-CoV samples.
Product Name:
MERS-CoV Nucleoprotein Monoclonal Antibody
SKU:
CAB21179
Size:
20μL, 100μL
Reactivity:
MERS-CoV
Clone Number:
ARC52592
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
Recombinant MERS-CoV Nucleoprotein Protein
Calculated MW:
45kDa
Observed MW:
47kDa(RecombinantProtein)
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
Purification Method
Affinity purification
Gene ID
14254601
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using MERS-CoV Nucleoprotein Rabbit mAb (CAB21179) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
