Anti-MET Antibody (CAB17366)

Antibody Name:	Anti-MET Antibody
Antibody SKU:	CAB17366
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human MET.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant protein of human MET.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4233
Uniprot:	P08581
Cellular Location:	Membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	85kDa/155kDa/157kDa
Observed MW:	170KDa

Synonyms:

MET, AUTS9, DFNB97, HGFR, RCCP2, c-Met

Background:

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

UniProt Protein Function:	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis.
NCBI Summary:	This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]
UniProt Code:	P08581
NCBI GenInfo Identifier:	251757497
NCBI Gene ID:	4233
NCBI Accession:	P08581.4
UniProt Secondary Accession:	P08581,O60366, Q12875, Q9UDX7, Q9UPL8, A1L467, B5A932 E7EQ94,
UniProt Related Accession:	P08581
Molecular Weight:	85,745 Da
NCBI Full Name:	Hepatocyte growth factor receptor
NCBI Synonym Full Names:	MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol:	MET
NCBI Official Synonym Symbols:	HGFR; AUTS9; RCCP2; c-Met; DFNB97
NCBI Protein Information:	hepatocyte growth factor receptor
UniProt Protein Name:	Hepatocyte growth factor receptor
UniProt Synonym Protein Names:	HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Protein Family:	C-methyltransferase
UniProt Gene Name:	MET

	Western blot analysis of extracts of various cell lines, using MET antibody (CAB17366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human lung cancer using MET Rabbit pAb (CAB17366) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MET Antibody (CAB0040)	Human Hepatocyte growth factor receptor (MET) ELISA Kit
Anti-Phospho-MET-Y1003 Antibody (CABP0076)	Met (Phospho-Tyr1234) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-MET-Y1349 Antibody (CABP0077)	Met Colorimetric Cell-Based ELISA Kit
Anti-Phospho-MET-Y1234 Antibody (CABP0078)	Met (Phospho-Tyr1356) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-MET-Y1235 Antibody (CABP0079)	Met (Phospho-Tyr1349) Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)