Anti-Phospho-MET-Y1234/1235 Antibody (CABP0533)

SKU:
CABP0533
€99 - €319

Description

Technical Manual

Anti-Phospho-MET-Y1234/1235 Antibody

Product Name:Anti-Phospho-MET-Y1234/1235 Antibody
Product SKU:CABP0533
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.

Immunogen:A synthetic phosphorylated peptide around Y1234 & Y1235 of human MET (NP_000236.2).
Sequence:

EYYS

Gene ID:4233
Swiss Prot:P08581
Synonyms:MET; AUTS9; DFNB97; HGFR; RCCP2; c-Met
Calculated MW:85kDa/155kDa/157kDa
Observed MW:156kDa
Reactivity:Human
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:HeLa
Cellular Location:Membrane, Secreted, Single-pass type I membrane protein

Product Images

Anti-Phospho-MET-Y1234/1235 Antibody (CABP0533)

Western blot - Phospho-MET-Y1234/1235 Rabbit pAb (CABP0533)

Uniprot Information

UniProt Protein Function:Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis.
NCBI Summary:This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016]
UniProt Code:P08581
NCBI GenInfo Identifier:251757497
NCBI Gene ID:4233
NCBI Accession:P08581.4
UniProt Secondary Accession:P08581,O60366, Q12875, Q9UDX7, Q9UPL8, A1L467, B5A932 E7EQ94,
UniProt Related Accession:P08581
Molecular Weight:85,745 Da
NCBI Full Name:Hepatocyte growth factor receptor
NCBI Synonym Full Names:MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol:MET
NCBI Official Synonym Symbols:HGFR; AUTS9; RCCP2; c-Met; DFNB97
NCBI Protein Information:hepatocyte growth factor receptor
UniProt Protein Name:Hepatocyte growth factor receptor
UniProt Synonym Protein Names:HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Protein Family:C-methyltransferase
UniProt Gene Name:MET
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Additional Information

Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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