Anti-MGMT Antibody (CAB0693)

Antibody Name:	Anti-MGMT Antibody
Antibody SKU:	CAB0693
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (XP_005252739.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (XP_005252739.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDKD CEMK RTTL DSPL GKLE LSGC EQGL HEIK LLGK GTSA ADAV EVPA PAAV LGGP EPLM QCTA WLNA YFHQ PEAI EEFP VPAL HHPV FQQE SFTR QVLW KLLK VVKF GEVI SYQQ LAAL AGNP KAAR AVGG AMRG NPVP ILIP CHRV VCSS GAVG NYSG GLAV KEWL LAHE GHRL GKPG LGGS SGLA GAWL KGAG ATSG SPPA GRN
Gene ID:	4255
Uniprot:	P16455
Cellular Location:	Nucleus
Calculated MW:	21kDa
Observed MW:	22kDa

Synonyms:

MGMT

Background:

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.

UniProt Protein Function:	MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.
UniProt Protein Details:	Protein type:EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis Chromosomal Location of Human Ortholog: 10q26 Cellular Component: nucleoplasm; membrane; nucleus Molecular Function:methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation
UniProt Code:	P16455
NCBI GenInfo Identifier:	127069
NCBI Gene ID:	4255
NCBI Accession:	P16455.1
UniProt Secondary Accession:	P16455,Q5VY78,
UniProt Related Accession:	P16455
Molecular Weight:	21,646 Da
NCBI Full Name:	Methylated-DNA--protein-cysteine methyltransferase
NCBI Synonym Full Names:	O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol:	MGMT
NCBI Protein Information:	methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
UniProt Protein Name:	Methylated-DNA--protein-cysteine methyltransferase
UniProt Synonym Protein Names:	6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
Protein Family:	Probable methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name:	MGMT
UniProt Entry Name:	MGMT_HUMAN

	Western blot analysis of extracts of HepG2 cells, using MGMT antibody (CAB0693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using MGMT antibody (CAB0693). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-MGMT Antibody (CAB0052)	Human MGMT ELISA Kit
Anti-MGMT Antibody (CAB11151)	Human Methylated-DNA--protein-cysteine methyltransferase (MGMT) ELISA Kit
MGMT Antibody (PACO06641)
MGMT Antibody (PACO10483)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)