Anti-MICA Antibody (CAB12622)

Antibody Name:	Anti-MICA Antibody
Antibody SKU:	CAB12622
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-307 of human MICA (NP_000238.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	K-562, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 24-307 of human MICA (NP_000238.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EPHS LRYN LTVL SWDG SVQS GFLT EVHL DGQP FLRC DRQK CRAK PQGQ WAED VLGN KTWD RETR DLTG NGKD LRMT LAHI KDQK EGLH SLQE IRVC EIHE DNST RSSQ HFYY DGEL FLSQ NLET KEWT MPQS SRAQ TLAM NVRN FLKE DAMK TKTH YHAM HADC LQEL RRYL KSGV VLRR TVPP MVNV TRSE ASEG NITV TCRA SGFY PWNI TLSW RQDG VSLS HDTQ QWGD VLPD GNGT YQTW VATR ICQG EEQR FTCY MEHS GNHS THPV PSGK VLVL QSHW
Gene ID:	100507436
Uniprot:	Q29983
Cellular Location:	Cell membrane, Cytoplasm, Single-pass type I membrane protein
Calculated MW:	31kDa/42kDa
Observed MW:	50kDa

Synonyms:

MICA, MIC-A, PERB11.1

Background:

This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: extracellular space; cell surface; integral to plasma membrane; cytoplasm Molecular Function:protein binding; beta-2-microglobulin binding; antigen binding; natural killer cell lectin-like receptor binding Biological Process: antigen processing and presentation; viral reproduction; natural killer cell mediated cytotoxicity; response to heat; cytolysis; defense response to bacterium; immune response to tumor cell; gamma-delta T cell activation; response to DNA damage stimulus; defense response to virus; T cell mediated cytotoxicity Disease: Psoriatic Arthritis, Susceptibility To; Psoriasis 1, Susceptibility To
NCBI Summary:	This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:	Q29983
NCBI GenInfo Identifier:	74740024
NCBI Gene ID:	100507436
NCBI Accession:	Q29983.1
UniProt Related Accession:	Q29983
Molecular Weight:
NCBI Full Name:	MHC class I polypeptide-related sequence A
NCBI Synonym Full Names:	MHC class I polypeptide-related sequence A
NCBI Official Symbol:	MICA
NCBI Official Synonym Symbols:	MIC-A; PERB11.1
NCBI Protein Information:	MHC class I polypeptide-related sequence A
UniProt Protein Name:	MHC class I polypeptide-related sequence A
Protein Family:	MICAL-like protein
UniProt Gene Name:	MICA
UniProt Entry Name:	MICA_HUMAN

	Western blot analysis of extracts of various cell lines, using MICA Antibody (CAB12622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human stomach using MICA antibody (CAB12622) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MICA Antibody (CAB1390)	Human MHC class I polypeptide-related sequence A (MICA) ELISA Kit
MICA Antibody (PACO10487)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)