The Human IFN-gamma ELISA (Small Sample Volume) is designed for the quantitative detection of Human IFN-gamma levels in various biological samples. Interferons (IFNs) are a family of secreted glycoproteins that constitute a critical arm of innate antiviral defense, inducing an antiviral state in neighboring cells, activating natural killer cells, and upregulating MHC class I antigen presentation. Type I IFNs (IFN-α/β) are rapidly produced in response to viral nucleic acids, while IFN-γ (Type II) is primarily secreted by T lymphocytes and NK cells to coordinate adaptive immune responses. IFNs are extensively studied in antiviral immunity, autoimmune diseases such as systemic lupus erythematosus, and as immunotherapeutic agents in cancer treatment. Assay Genie's Human IFN-gamma ELISA (Small Sample Volume) offers exceptional sensitivity and specificity with a sensitivity of 14.06 pg/mL, ensuring reliable and reproducible results across a broad range of sample types including serum, plasma and other biological fluids. Manufactured under stringent quality control standards, this ELISA kit provides robust performance and ease of use, making it an outstanding choice for both research and clinical applications. Trust Assay Genie's Human IFN-gamma ELISA (Small Sample Volume) for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human IFN-gamma ELISA (Small Sample Volume)
SKU:
AEES03466
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
14.06 pg/mL
Detection range:
23.44-1500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
