Anti-MKL1 Antibody (CAB8504)

Antibody Name:	Anti-MKL1 Antibody
Antibody SKU:	CAB8504
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	TE-1, HeLa, Mouse lung, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPPL KSPA AFHE QRRS LERA RTED YLKR KIRS RPER SELV RMHI LEET SAEP SLQA KQLK LKRA RLAD DLNE KIAQ RPGP MELV EKNI LPVE SSLK EAII VGQV NYPK VADS SSFD EDSS DALS PEQP ASHE SQGS VPSP LEAR VSEP LLSA TSAS PTQV VSQL PMGR DSRE MLFL AEQP PLPP PPLL PPSL TNGT TIPT AKST PTLI KQSQ PKSA SEKS QRSK KAKE LKPK VKKL KYHQ YIPP DQKQ DRGA PPMD SSYA KILQ QQQL FLQL QILN QQQQ
Gene ID:	57591
Uniprot:	Q969V6
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	98kDa
Observed MW:	145kDa

Synonyms:

MKL1, BSAC, MAL, MRTF-A

Background:

The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MKL1: Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation. Forms with SCAI and SRF a nuclear ternary complex which binds the CArG consensus motif (CArG box) on DNA via SRF. Interacts with ACTB; interaction with ACTB prevents interaction with SCAI. Interacts with MKL2. Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.
UniProt Protein Details:	Protein type:Oncoprotein; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 22q13 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:actin binding; actin monomer binding; leucine zipper domain binding; protein binding; transcription coactivator activity Biological Process: positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation
NCBI Summary:	The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:	Q969V6
NCBI GenInfo Identifier:	32363202
NCBI Gene ID:	57591
NCBI Accession:	Q969V6.1
UniProt Secondary Accession:	Q969V6,Q8TCL1, Q96SC5, Q96SC6, Q9P2B0,
UniProt Related Accession:	Q969V6
Molecular Weight:	98,919 Da
NCBI Full Name:	MKL/myocardin-like protein 1
NCBI Synonym Full Names:	megakaryoblastic leukemia (translocation) 1
NCBI Official Symbol:	MKL1
NCBI Official Synonym Symbols:	MAL; BSAC; MRTF-A
NCBI Protein Information:	MKL/myocardin-like protein 1
UniProt Protein Name:	MKL/myocardin-like protein 1
UniProt Synonym Protein Names:	Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; Myocardin-related transcription factor A; MRTF-A
Protein Family:	MKL/myocardin-like protein
UniProt Gene Name:	MKL1
UniProt Entry Name:	MKL1_HUMAN

	Western blot analysis of extracts of various cell lines, using MKL1 antibody (CAB8504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human tonsil using MKL1 antibody (CAB8504) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using Anti-MKL1 Polyclonal Antibody (CAB8504) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-MKL1 Antibody (CAB12598)

Anti-MKL1 Antibody (CAB14212)

MKL1 Antibody (PACO01111)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)