Anti-MLKL Antibody (CAB13451)

Antibody Name:	Anti-MLKL Antibody [KO Validated]
Antibody SKU:	CAB13451
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of mouse MLKL

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, NCI-H460, Mouse liver, Rat testis

Immunogen:	Recombinant protein of mouse MLKL
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	74568
Uniprot:	Q9D2Y4
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	53kDa/54kDa
Observed MW:	54kDa

Synonyms:

MLKL, hMLKL

Background:

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

UniProt Protein Function:	MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique familyCellular Component: membrane; cytoplasm; plasma membraneMolecular Function: transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; protein kinase binding; ATP binding; protein kinase activityBiological Process: programmed cell death; protein amino acid phosphorylation
UniProt Protein Details:
NCBI Summary:	This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Code:	Q9D2Y4
NCBI GenInfo Identifier:	81916976
NCBI Gene ID:	74568
NCBI Accession:	Q9D2Y4.1
UniProt Secondary Accession:	Q9D2Y4,Q8CIJ5
UniProt Related Accession:	Q9D2Y4
Molecular Weight:	54kDa
NCBI Full Name:	Mixed lineage kinase domain-like protein
NCBI Synonym Full Names:	mixed lineage kinase domain-like
NCBI Official Symbol:	Mlkl
NCBI Official Synonym Symbols:	9130019I15Rik
NCBI Protein Information:	mixed lineage kinase domain-like protein; mlkl
UniProt Protein Name:	Mixed lineage kinase domain-like protein
UniProt Synonym Protein Names:
Protein Family:	Mixed lineage kinase domain-like protein
UniProt Gene Name:	Mlkl
UniProt Entry Name:	MLKL_MOUSE

	Western blot analysis of extracts from normal (control) and MLKL knockout (KO) HeLa cells, using MLKL antibody (CAB13451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using MLKL antibody (CAB13451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of Mouse liver, using MLKL antibody (CAB13451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of extracts of Rat testis, using MLKL antibody (CAB13451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human breast cancer using MLKL antibody (CAB13451) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MLKL Antibody (CAB5579)	Mouse Mixed lineage kinase domain-like protein (Mlkl) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)