Anti-MLKL Antibody (CAB17312)

Antibody Name:	Anti-MLKL Antibody
Antibody SKU:	CAB17312
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of mouse MLKL

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, NCI-H460

Immunogen:	Recombinant protein of mouse MLKL
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	197259
Uniprot:	Q8NB16
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	53kDa/54kDa
Observed MW:	54kDa

Synonyms:

hMLKL, MLKL

Background:

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.

UniProt Protein Function:	MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; TKL-Unique family Chromosomal Location of Human Ortholog: 16q23.1 Cellular Component: cytoplasm; cytosol; plasma membrane Molecular Function:ATP binding; protein binding; protein complex binding; protein kinase activity; signal transducer activity Biological Process: protein amino acid phosphorylation
NCBI Summary:	This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
UniProt Code:	Q8NB16
NCBI GenInfo Identifier:	74762545
NCBI Gene ID:	197259
NCBI Accession:	Q8NB16.1
UniProt Secondary Accession:	Q8NB16,Q8N6V0, A6NCE4,
UniProt Related Accession:	Q8NB16
Molecular Weight:	54kDa
NCBI Full Name:	Mixed lineage kinase domain-like protein
NCBI Synonym Full Names:	mixed lineage kinase domain like pseudokinase
NCBI Official Symbol:	MLKL
NCBI Official Synonym Symbols:	hMLKL
NCBI Protein Information:	mixed lineage kinase domain-like protein
UniProt Protein Name:	Mixed lineage kinase domain-like protein
UniProt Gene Name:	MLKL

	Western blot analysis of extracts of various cell lines, using MLKL antibody (CAB17312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver cancer using MLKL Rabbit pAb (CAB17312) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of C6 cells using MLKL Rabbit pAb (CAB17312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using MLKL Rabbit pAb (CAB17312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using MLKL Rabbit pAb (CAB17312) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-MLKL Antibody (CAB11458)	Human MLKL (mixed lineage kinase domain-like) ELISA Kit (HUFI03458)
Anti-MLKL Antibody [KO Validated] (CAB19685)	Human Mixed lineage kinase domain-like protein (MLKL) ELISA Kit
Anti-Phospho-MLKL-T357/S358/S360 Antibody (CABP0949)
MLKL Antibody (PACO01071)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)