Anti-MT1-MMP Antibody (CAB2549)

Antibody Name:	Anti-MT1-MMP Antibody
Antibody SKU:	CAB2549
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human MT1-MMP

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, NCI-H460, Mouse liver

Immunogen:	Recombinant protein of human MT1-MMP
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4323
Uniprot:	P50281
Cellular Location:	Cytoplasm, Melanosome, Membrane, Single-pass type I membrane protein
Calculated MW:	65kDa
Observed MW:	46-70kDa

Synonyms:

MMP14, MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, WNCHRS, MT-MMP1

Background:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.

UniProt Protein Function:	MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.
UniProt Protein Details:	Protein type:EC 3.4.24.80; Protease; Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: extracellular matrix; focal adhesion; Golgi lumen; integral to plasma membrane; cytoplasm; melanosome; plasma membrane Molecular Function:integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; negative regulation of focal adhesion formation; male gonad development; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; regulation of transcription, DNA-dependent; branching morphogenesis of a tube; ovarian follicle development; response to estrogen stimulus; response to mechanical stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; response to oxidative stress; angiogenesis; astrocyte cell migration; lung development; positive regulation of cell migration; endochondral ossification Disease: Winchester Syndrome
NCBI Summary:	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
UniProt Code:	P50281
NCBI GenInfo Identifier:	317373419
NCBI Gene ID:	4323
NCBI Accession:	P50281.3
UniProt Secondary Accession:	P50281,Q6GSF3, Q92678, A8K5L0,
UniProt Related Accession:	P50281
Molecular Weight:	65,894 Da
NCBI Full Name:	Matrix metalloproteinase-14
NCBI Synonym Full Names:	matrix metallopeptidase 14 (membrane-inserted)
NCBI Official Symbol:	MMP14
NCBI Official Synonym Symbols:	MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
NCBI Protein Information:	matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase
UniProt Protein Name:	Matrix metalloproteinase-14
UniProt Synonym Protein Names:	MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	MMP14
UniProt Entry Name:	MMP14_HUMAN

	Western blot analysis of extracts of various cell lines, using MT1-MMP antibody (CAB2549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using MT1-MMP antibody (CAB2549) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using MT1-MMP antibody (CAB2549) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MMP14 Antibody (CAB15691)	Human Matrix metalloproteinase-14 (MMP14) ELISA Kit
Anti-MT1-MMP Antibody (CAB0067)	MMP-14 Colorimetric Cell-Based ELISA Kit
Cleaved-MMP14 (Y112) Antibody (PACO00033)
MMP14 Antibody (PACO04347)
MMP14 Antibody (PACO10523)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)