Anti-MMP2 Antibody (CAB6247)

Antibody Name:	Anti-MMP2 Antibody
Antibody SKU:	CAB6247
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-280 of human MMP2 (NP_004521.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A375, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 40-280 of human MMP2 (NP_004521.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DVAP KTDK ELAV QYLN TFYG CPKE SCNL FVLK DTLK KMQK FFGL PQTG DLDQ NTIE TMRK PRCG NPDV ANYN FFPR KPKW DKNQ ITYR IIGY TPDL DPET VDDA FARA FQVW SDVT PLRF SRIH DGEA DIMI NFGR WEHG DGYP FDGK DGLL AHAF APGT GVGG DSHF DDDE LWTL GEGQ VVRV KYGN ADGE YCKF PFLF NGKE YNSC TDTG RSDG FLWC STTY NFEK DGKY GFCP HEAL F
Gene ID:	4313
Uniprot:	P08253
Cellular Location:	Cytoplasm, Membrane, Mitochondrion, Nucleus, Secreted, extracellular matrix, extracellular space
Calculated MW:	65kDa/68kDa/73kDa
Observed MW:	73kDa

Synonyms:

CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1, MMP2

Background:

This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-\|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.
UniProt Protein Details:	Protein type:Secreted; Motility/polarity/chemotaxis; Cell development/differentiation; Protease; Apoptosis; EC 3.4.24.24; Secreted, signal peptide Chromosomal Location of Human Ortholog: 16q12.2 Cellular Component: extracellular space; proteinaceous extracellular matrix; sarcomere; mitochondrion; extracellular region; plasma membrane; nucleus Molecular Function:protein binding; zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity Biological Process: extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; intramembranous ossification; cellular protein metabolic process; positive regulation of innate immune response; ephrin receptor signaling pathway; response to hypoxia; angiogenesis; proteolysis; blood vessel maturation; embryo implantation Disease: Multicentric Osteolysis, Nodulosis, And Arthropathy
NCBI Summary:	This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
UniProt Code:	P08253
NCBI GenInfo Identifier:	116856
NCBI Gene ID:	4313
NCBI Accession:	P08253.2
UniProt Secondary Accession:	P08253,Q9UCJ8, B2R6U1, B4DWH3, E9PE45,
UniProt Related Accession:	P08253
Molecular Weight:	68,831 Da
NCBI Full Name:	72 kDa type IV collagenase
NCBI Synonym Full Names:	matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
NCBI Official Symbol:	MMP2
NCBI Official Synonym Symbols:	CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
NCBI Protein Information:	72 kDa type IV collagenase; gelatinase A; 72 kDa gelatinase; collagenase type IV-A; neutrophil gelatinase; matrix metalloproteinase-2; matrix metalloproteinase-II
UniProt Protein Name:	72 kDa type IV collagenase
UniProt Synonym Protein Names:	72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1Cleaved into the following chain:PEX
Protein Family:	Matrix metalloproteinase
UniProt Gene Name:	MMP2
UniProt Entry Name:	MMP2_HUMAN

	Western blot analysis of extracts of various cell lines, using MMP2 antibody (CAB6247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human appendix using MMP2 antibody (CAB6247) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MMP2 Antibody (CAB0317)	Human MMP-2 (Matrix Metalloproteinase 2) CLIA Kit (HUES00822)
Anti-MMP2 Antibody (CAB11144)	Human 72 kDa type IV collagenase (MMP2) ELISA Kit
Anti-MMP2 Antibody [KO Validated] (CAB19080)	MMP-2 Colorimetric Cell-Based ELISA Kit
MMP2 Antibody (PACO06646)
MMP2 Antibody (PACO10525)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)