The Monkey CD30 (Lymphocyte Activation Antigen CD30) ELISA Kit is a cutting-edge tool that is specifically designed for the precise detection of CD30 levels in monkey serum, plasma, and cell culture supernatants. This innovative kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are ideal for a wide range of research applications. CD30, also known as lymphocyte activation antigen CD30, is a vital protein that plays a crucial role in the activation and regulation of lymphocytes, particularly in immune response processes. Dysregulation of CD30 has been linked to various diseases and conditions, including autoimmune disorders, inflammatory diseases, and certain types of cancer.
Thus, the Monkey CD30 ELISA Kit serves as a valuable tool for researchers studying these conditions and exploring potential therapeutic interventions. With its advanced technology and superior performance, the Monkey CD30 (Lymphocyte Activation Antigen CD30) ELISA Kit is a reliable and indispensable resource for scientists in the field of immunology and related disciplines. Elevate your research endeavors with this state-of-the-art kit that delivers precise and consistent results every time.
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-102%
85-101%
85-100%
EDTA Plasma (n = 5)
87-105%
87-101%
95-96%
Heparin Plasma (n = 5)
96-104%
89-95%
88-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-102
95
EDTA Plasma (n = 5)
91-105
98
Heparin Plasma (n = 5)
92-103
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
