Description
Mono-Lux Luciferase Assay System (ASRV00015)
The Mono-Lux Luciferase Assay System is a homogeneous, single-reagent solution designed for the ultrahigh sensitivity and stable quantitation of firefly luciferase reporter gene expression in mammalian cells. This robust system is ideally suited for high- and ultrahigh-throughput (UHTS) applications and batch processing of 96- and 384-well plates, delivering a stable optical signal upon direct addition to cultured cells. The Mono-Lux system contains high-purity Luciferin and an optimized reaction reagent formulation. This proprietary chemistry provides enhanced stability, greater tolerance to environmental factors and sample components, and results in less peculiar smell compared to standard luciferase assay reagents.
| Product Name: | Mono-Lux Luciferase Assay System |
| SKU: | ASRV00015 |
| Size: | 10ml, 10×10ml, 100ml |
| Detection Principle: | Luminescence |
| Step | Protocol |
| 1 | Remove the cell culture plate to be tested from the incubator and equilibrate at room temperature for 30 min. |
| 2 | Add equal amount of Mono-Lux Luciferase Detection Reagent which is equal to the volume of the cell culture to be tested. For example, when using a 96-well culture plate, add 100 μl Mono-Lux Luciferase Detection Reagent to 100 μl cell culture to be tested. |
| 3 | Incubate at room temperature for at least 3 minutes to lyse the cells. |
| 4 | Detect luminescence. |
| Step | Protocol |
| Thawing | Incubate the Mono-Lux Luciferase Assay Buffer at 2 to 8℃ or room temperature to thaw. The product can also be incubated at 22℃ in a water bath but the temperature should not exceed 25℃. |
| Preparation of Mono-Lux Luciferase Detection Reagent | Add the entire bottle of thawed Mono-Lux Luciferase Buffer into the Mono-Lux Luciferase Substrate. Gently invert and mix it well for 3 to 5 times until the substrate is dissolved thoroughly. |
| Equilibrate to room temperature | Equilibrate the Mono-Lux Luciferase Detection Reagent to room temperature before use. |
| Mix | Gently invert 5 times before use, to mix the solution evenly. |
| Product | Code | Contents |
| Mono-Lux Luciferase Assay System (10ml) | ASRV00015-10 |
Mono-Lux Luciferase Assay Buffer (10ml) Mono-Lux Luciferase Assay Substrate (lyophilized) 1 Vial |
| Mono-Lux Luciferase Assay System (10x10ml) | ASRV00015-10x10 |
Mono-Lux Luciferase Assay Buffer (10x10ml) Mono-Lux Luciferase Assay Substrate (lyophilized) 10 Vials |
| Mono-Lux Luciferase Assay System (100ml) | ASRV00015-100 |
Mono-Lux Luciferase Assay Buffer (100ml) Mono-Lux Luciferase Assay Substrate (lyophilized) 1 Vial |
