The MonoMethyl-Histone H3-K36 Antibody (CAB2364) is a high-quality antibody developed for reliable detection and analysis of target proteins. Histone H3 lysine 36 monomethylation is a crucial modification involved in transcriptional activation and elongation processes. This antibody, raised in rabbits, specifically targets the monomethylated form of histone H3 at lysine 36 and is highly reactive in human samples.Validated for use in Western blot applications, the Monomethyl Histone H3 (K36) Polyclonal Antibody (CAB2364) allows for the detection and analysis of this epigenetic mark in various cell types. Its specificity and sensitivity make it an ideal tool for research in the fields of epigenetics, chromatin biology, and gene regulation.
This antibody is validated for use in WB, IF/ICC, ChIP, ChIP-seq, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H3-K36 Antibody
SKU:
CAB2364
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBIF/ICCChIPChIP-seqELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2764324
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-Histone H3-K36 Rabbit pAb (CAB2364) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-K36 antibody (CAB2364).
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (CAB2364) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
