The Mouse AChE (Acetylcholinesterase) ELISA Kit is specifically designed for the accurate and reliable quantification of AChE levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Acetylcholinesterase is an essential enzyme involved in the regulation of neurotransmission by breaking down the neurotransmitter acetylcholine. Dysregulation of AChE activity has been implicated in various neurological disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With its easy-to-use protocol and accurate performance, the Mouse AChE ELISA Kit is a valuable tool for researchers looking to investigate the role of AChE in normal physiological processes and disease states in mouse models.
Product Name:
Mouse AChE (Acetylcholinesterase) ELISA Kit
SKU:
MOES01775
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
