Mouse Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) ELISA Kit
The Mouse ARPC4 ELISA Kit is specifically designed for the precise quantification of mouse Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) levels in various sample types, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable tool for researchers in a variety of fields.ARPC4 is a critical component of the actin-related protein 2/3 complex, which plays a key role in actin filament nucleation and branching, essential processes in cellular motility and shape.
Dysregulation of ARPC4 has been implicated in various diseases, including cancer and immune disorders, making it a promising target for therapeutic interventions.By utilizing the Mouse ARPC4 ELISA Kit, researchers can gain valuable insights into the role of ARPC4 in disease pathogenesis and identify potential therapeutic strategies for targeting this protein. This kit is a valuable asset for any laboratory conducting research on actin dynamics and its implications in disease development and progression.
Product Name:
Mouse Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
