The Mouse Albumin (ALB) ELISA Kit is meticulously crafted for the precise quantification of Albumin levels in diverse biological samples. Albumin, a vital protein produced in the liver, serves essential roles in maintaining osmotic pressure, transporting molecules, and contributing to various physiological functions. This ELISA kit delivers unparalleled sensitivity and specificity to ensure accurate measurement of Albumin levels, enabling researchers to explore aspects related to liver function, kidney health, and metabolic processes. With stringent quality control measures, this kit guarantees reliable performance and reproducible results, offering a valuable tool for scientific investigations and clinical research.
Product Name:
Mouse ALB (Albumin) ELISA Kit
SKU:
AEES00227
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 ng/mL
Detection range:
62.5-4000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
