The Mouse Alcohol Dehydrogenase 7 (ADH7) ELISA Kit is specifically designed for the precise quantification of ADH7 levels in mouse serum, plasma, and tissue lysate samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ADH7 is an important enzyme involved in alcohol metabolism, playing a key role in the breakdown of ethanol in the liver. This enzyme is essential for the detoxification process and can impact individual differences in alcohol metabolism and susceptibility to alcohol-related diseases.
By measuring ADH7 levels in biological samples, researchers can gain valuable insights into alcohol metabolism, liver function, and potential implications for alcohol-related health issues. This ELISA kit provides a convenient and reliable tool for studying the role of ADH7 in alcohol metabolism and related physiological processes, paving the way for new therapeutic strategies and interventions.
Product Name:
Mouse Alcohol Dehydrogenase 7 (ADH7) ELISA Kit
Product Code:
MODL00026
Size:
96 Assays
Target:
ADH7
Synonyms:
Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase
Detection Method:
Competition
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
