Description
Mouse ALOX12E Recombinant Protein (N-His)
Mouse ALOX12E Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Thr112, with an N-terminal His tag. Predicted molecular weight: 14.47 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Polyunsaturated fatty acid (12S)/(13S)-lipoxygenase, epidermal-type is a ~75 kDa protein. Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. For research use only.
Shows increasing catalytic activity within the series arachidonic acid < 5,8,11-eicosatrienoic acid < linoleic acid < 8,11,14-eicosatrienoic acid.
| Product Name: | Mouse ALOX12E Recombinant Protein (N-His) |
| SKU: | GXRP13421 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | 12-LOX-e, Alox12-ps2, Alox12e, Aloxe, Arachidonate (12S)-lipoxygenase, epidermal-type, EC:1.13.11.-, EC:1.13.11.31, Linoleate (13S)-lipoxygenase, Polyunsaturated fatty acid (12S)/(13S)-lipoxygenase, epidermal-type, e(12S)-LOX |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Thr112 |
| Calculated MW: | 14.47 kDa |
| UniProt: | P55249 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
