The Mouse Alpha-2 Macroglobulin (A2M) ELISA Kit is a powerful tool for the precise measurement of alpha-2 macroglobulin levels in mouse samples. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Alpha-2 macroglobulin is a key protein involved in immune response and inflammation regulation. It acts as a major broad-spectrum protease inhibitor, playing a vital role in maintaining homeostasis in the body.
Dysregulation of alpha-2 macroglobulin has been linked to various diseases such as cancer, arthritis, and Alzheimer's, highlighting its importance as a potential diagnostic and therapeutic target.With the Mouse Alpha-2 Macroglobulin (A2M) ELISA Kit, researchers can confidently study the role of alpha-2 macroglobulin in disease progression and therapeutic interventions, leading to advancements in the field of biomedical research.
Product Name:
Mouse Alpha-2-Macroglobulin (a2M) ELISA Kit
Product Code:
MODL00004
Size:
96 Assays
Target:
a2M
Synonyms:
A2MG, A2mg, CPAMD5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Detection Method:
Sandwich
Range:
9.375-600ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
