Description
Mouse ALPL Recombinant Protein (C-His)
Mouse ALPL Recombinant Protein (C-His) is a recombinant protein expressed in mammalian cells, spanning Phe18-Ser502, with a C-terminal His tag. Predicted molecular weight: 56.48 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Alkaline phosphatase, tissue-nonspecific isozyme is a ~57 kDa protein. Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. For research use only.
Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state; it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner.
| Product Name: | Mouse ALPL Recombinant Protein (C-His) |
| SKU: | GXRP12649 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | ALPL, AP-TNAP, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase, tissue-nonspecific isozyme, EC:3.1.3.1, EC:3.9.1.1, Phosphoamidase, Phosphocreatine phosphatase, TNS-ALP, TNSALP |
| Tag: | C-His |
| Expression Host: | Mammalian cells |
| Protein Length: | Phe18-Ser502 |
| Calculated MW: | 56.48 kDa |
| UniProt: | P09242 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
