Mouse Angiopoietin Like Protein 3 (ANGPTL3) ELISA Kit
The Mouse ANGPTL3 (Angiopoietin-like Protein 3) ELISA Kit is a powerful tool for the precise measurement of ANGPTL3 levels in mouse serum, plasma, and cell culture supernatants. With its reliable accuracy and specificity, this kit is perfect for various research applications.ANGPTL3 is a key protein involved in lipid metabolism and plays a crucial role in regulating triglyceride levels and cholesterol homeostasis. Dysregulation of ANGPTL3 has been linked to various metabolic disorders, making it a valuable biomarker for studying conditions such as obesity, cardiovascular diseases, and diabetes, and for developing potential therapeutic strategies.
This ELISA kit offers high sensitivity and reproducibility, allowing researchers to obtain consistent and dependable results. It is an essential tool for investigating the role of ANGPTL3 in metabolic pathways and exploring its potential as a target for novel interventions.
Product Name:
Mouse Angiopoietin Like Protein 3 (ANGPTL3) ELISA Kit
Product Code:
MODL00073
Size:
96 Assays
Target:
ANGPTL3
Synonyms:
ANGPT5, ANG-5, Angiopoietin 5, Angiopoietin-related protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
