The Mouse APLN (Apelin) ELISA Kit is a reliable tool for measuring apelin levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for various research applications.Apelin is a peptide hormone that plays a key role in regulating cardiovascular function, energy metabolism, and fluid homeostasis. Dysregulation of apelin expression has been linked to diseases such as obesity, diabetes, and cardiovascular disorders, making it an important biomarker for studying these conditions and developing new treatment strategies. Overall, the Mouse APLN ELISA Kit offers researchers a valuable tool for investigating the physiological and pathological roles of apelin in mouse models, providing insights into its potential as a therapeutic target.
Product Name:
Mouse APLN (Apelin) ELISA Kit
SKU:
MOES01702
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
75 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
