Description
Mouse ASAH2 Recombinant Protein (N-His)
Mouse ASAH2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Glu641-Thr756, with an N-terminal His tag. Predicted molecular weight: 15.77 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Neutral ceramidase (ASAH2) is a ~83 kDa protein. Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. For research use only.
Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells.
| Product Name: | Mouse ASAH2 Recombinant Protein (N-His) |
| SKU: | GXRP05883 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Mouse |
| Synonyms: | Acylsphingosine deacylase 2, Neutral ceramidase, NCDase, N-acylsphingosine amidohydrolase 2, Asah2, N-CDase |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Glu641-Thr756 |
| Calculated MW: | 15.77 kDa |
| UniProt: | Q9JHE3 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
