The Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit is designed for the accurate and precise measurement of BALP levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for various research applications.BALP is a key enzyme found in bone tissue that plays a critical role in bone mineralization and turnover. Monitoring BALP levels can provide valuable insights into bone health and metabolism, making it a valuable biomarker for studying bone-related disorders and evaluating potential treatments.Overall, the Mouse BALP CLIA Kit is a valuable tool for researchers seeking to analyze bone alkaline phosphatase levels in mouse models, providing essential data for further understanding bone physiology and pathology.
Product Name:
Mouse BALP (Bone Alkaline Phosphatase) CLIA Kit
SKU:
MOES00127
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
