The Mouse BDNF (Brain-Derived Neurotrophic Factor) CLIA Kit is specially designed for the precise measurement of BDNF levels in mouse serum, plasma, and tissue extracts. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research purposes.BDNF is a key neurotrophic factor that plays a vital role in regulating neuronal growth, development, and survival. Dysregulation of BDNF is associated with various neurological disorders, including Alzheimer's disease, depression, and schizophrenia, highlighting its importance as a biomarker for studying these conditions and exploring therapeutic interventions. With its advanced technology and user-friendly design, the Mouse BDNF CLIA Kit from Assaygenie is an invaluable tool for researchers seeking to unravel the complexities of neurobiology and pave the way for innovative solutions in neuroscience and clinical medicine.
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
